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KMID : 0357920090430020164
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Korean Journal of Pathology
2009 Volume.43 No. 2 p.164 ~ p.170
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The Cytology for Leukemic Cells in Cerebrospinal Fluid; Comparison of Conventional Cytology with Liquid-Based Cytology
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Yoo Chang-Young
Lee Youn-Soo
Kang Chang-Suk
Shim Sang-In
Lee Kyo-Young
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Abstract
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Background: The cytological examination of cerebrospinal fluid (CSF) using conventional cytology with a cytocentrifuge (cytospin) is an important method for evaluating the involvement of leukemia in the CNS. Liquid-based cytology (LBC) is now a widely used cytological method not only for gynecological and non-gynecological specimens, but its application to CSF for the identification of leukemic cell has not yet been reported. In this study, we tried to compare conventional cytology with using a cytospin with LBC and Papanicolaou (Pap) staining. We also examined the modified LBC with Wright staining to assess whether this modified method can be useful for diagnosing Leukemia.
Methods: We studied 30 cases of CSF that were obtained from 16 patients, including 17 cases of acute myeloid leukemia, 12 cases of acute lymphoblastic leukemia and 1 case of diffuse large B cell lymphoma. We applied conventional cytology with a cytocentrifuge (cytospin), LBC with Pap staining and modified LBC with Wright staining.
Results: The morphological features of the LBC with Pap staining showed difficulty for interpretation when compared with conventional cytology with a cytospin, and mainly because of cellular shrinkage. The modified LBC with Wright staining showed good morphological features.
Conclusions: We suggest that modified LBC with Wright staining may be useful for examining CSF.
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KEYWORD
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Cerebrospinal fluid, Liquid-based cytology, Cytospin, Wright staining, Papanico-laoustaining
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